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Subsequently, citrate is converted to isocitrate, which is then catalyzed by the rate-limiting enzyme isocitrate dehydrogenase to produce -ketoglutarate

37 Average saliva secretion is about 1500 mL per day, about 10 mL/h, variably

Observational study - a human research study in which no experimental intervention or treatment is applied, and participants are simply observed over time Oxidative stress - a situation in which the production of reactive oxygen species exceeds the ability of an organism to eliminate or neutralize them Placebo - a chemically inactive substance Plaque - a deposit of fat, cholesterol, immune cells, fibrin (a clotting protein), and other substances that forms inside arterial walls Randomized controlled trial - a human research study in which participants are assigned by chance alone to receive either an experimental agent (the treatment group) or a placebo (the control group) Reactive oxygen species (ROS) - highly unstable oxygen-containing compounds that react easily with nearby cellular structures, potentially causing damage Test tube - a research experiment performed in a test tube, culture dish, or other artificial environment outside of a living organism

Two tubes for each isolate were prepared and sub MIC of vitamin C was added to one of them