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Methods Reagents and tools table Maintenance of human ESC lines HES3 NKX2-5 eGFP/w hESCs (Elliott et al, 2011) and gene-edited derivatives were maintained on a layer of mitotically-inactivated mouse embryonic fibroblasts (MEFs) in DMEM/F12-based medium containing 20% (v/v) KnockOut Serum Replacement (Gibco), 100 mM non-essential amino acids (Gibco), 2 mM GlutaMAX (Gibco), 0.1 mM -mercaptoethanol (Gibco) and 10 ng/ml bFGF (Miltenyi Biotech)