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To circumvent this limitation, cells developed two NADH shuttles: the malate/aspartate shuttle and the glycerol-3-phosphate shuttle (35, 65, 106)

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In addition to TCGA, we examined GLO expression in other published NSCLC proteomic studies 16,17 and observed similar effects as in our study: compared to healthy tissue, both GLO1 and GLO2 were prominently downregulated, while hypoxic targets were all upregulated in tumor (Fig

Synthetic biology outside the cell: linking computational tools to cell-free systems